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- Title
Improved Brilliant Blue G Staining of the Internal Limiting Membrane with Sharp Cut Filters of a Novel Viewing Filter System.
- Authors
Enaida, Hiroshi; Yoshida, Shigeo; Nakao, Shintaro; Ikeda, Yasuhiro; Hachisuka, Yoshiyuki; Oshima, Yusuke; Kadonosono, Kazuaki; Ueno, akifumi; Ishibashi, Tatsuro
- Abstract
Purpose: We developed a new artificial image enhancement system aimed at intraoperative visibility improvement as a clinical prototype. We examined each optical characteristic and change in intraoperative visibility using brilliant blue G (BBG) staining with various sharp cut filters (SCFs). Method: This was a retrospective and observational study. The system was composed of several filters attached to the operating microscope. Six eyes from 6 patients who presented with macular hole and underwent surgery using this system were studied. As a clinical examination, the intraoperative visibility of BBG staining intensities was compared for 4 kinds of SCFs during vitrectomy. Quantitative evaluation was calculated using the International Commission on Illumination 1976 (L*, a*, b*) color space (CIELAB) method. Furthermore, we evaluated each optical characteristic of 4 types of SCFs using extracted porcine eyes and a spectroradiometer as a clinical simulation. Results: Suitable filter selection was possible for this system. The observed color tone and spectral irradiance changes with SCF insertion changed dynamically. In macular hole cases, the color intensities between BBG-stained and nonstained areas were improved using SCF-455 and SCF-520, which was statistically significant (p < 0.05) by CIELAB. Conclusion: The system improved BBG staining intensity with the use of selective SCFs. © 2013 S. Karger AG, Basel
- Subjects
STAINS &; staining (Microscopy); FILTERS &; filtration; SPECTRAL irradiance; RADIANT intensity; SPECTRORADIOMETER
- Publication
Ophthalmologica, 2013, Vol 230, p27
- ISSN
0030-3755
- Publication type
Article
- DOI
10.1159/000353868