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- Title
Rapid isolation of plant peroxidase. Purification of peroxidase a from Petunia.
- Authors
van den Berg, Bartel M.; van Huystee, Robert B.
- Abstract
A rapid isolation procedure was developed for purification of peroxidase a from Petunia hybrida. Rapid isolation was possible since about 15% of the extracellular protein from stem tissue obtained by vacuum infiltration followed by centrifugation of the tissue represents peroxidase. Purification of peroxidase a from intercellular fluid was achieved by two acetone precipitation steps followed by DEAE-cellulose chromatography. Three different forms of peroxidase were dated from DEAE-cellulose at different NaCl concentrations. Isoelectric focusing showed, however, a pI of 3.8 for all three forms of peroxidase a. Only part of the peroxidase a enzymes bound to Concanavalin A indicating heterogeneity in the carbohydrate part. Homology of peroxidase a to the peroxidase G1 group front tobacco is discussed.
- Subjects
EXTRACELLULAR matrix; PETUNIAS; PEROXIDASE; CHROMATOGRAPHIC analysis; CELLULOSE; SALT
- Publication
Physiologia Plantarum, 1984, Vol 60, Issue 3, p299
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1984.tb06066.x