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- Title
Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner.
- Authors
Kida, Hiroshi; Nishimura, Koyo; Ogawa, Koki; Watanabe, Akiko; Feril, Loreto B.; Irie, Yutaka; Endo, Hitomi; Kawakami, Shigeru; Tachibana, Katsuro
- Abstract
Recent research has revealed that nanobubbles (NBs) can be an effective tool for gene transfection in conjunction with therapeutic ultrasound (US). However, an approach to apply commercially available hand-held diagnostic US scanners for this purpose has not been evaluated as of now. In the present study, we first compared in vitro , the efficiency of gene transfer (pCMV-Luciferase) with lipid-based and albumin-based NBs irradiated by therapeutic US (1MHz, 5.0 W/cm2) in oral squamous carcinoma cell line HSC-2. Secondly, we similarly examined if gene transfer in mice is possible using a clinical hand-held US scanner (2.3MHz, MI 1.0). Results showed that lipid-based NBs induced more gene transfection compared to albumin-based NBs, in vitro. Furthermore, significant gene transfer was also obtained in mice liver with lipid-based NBs. Sub-micro sized bubbles proved to be a powerful gene transfer reagent in combination with conventional hand-held ultrasonic diagnostic device.
- Subjects
GENETIC transformation; SCANNING systems; DIAGNOSTIC ultrasonic imaging; ULTRASONIC equipment; SQUAMOUS cell carcinoma; POCKET computers; GENE transfection
- Publication
Frontiers in Pharmacology, 2020, p1
- ISSN
1663-9812
- Publication type
Article
- DOI
10.3389/fphar.2020.00363