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- Title
Toxicity of repeated intravenous injection of gene therapeutics for X-CGD in mice.
- Authors
Lee, Y. M.; Choi, W. H.; Kim, Y. B.; Ha, C. S.; Song, C. W.; Lee, M.; Joo, C. W.; Hong, Y.; Ho, S. H.; Kim, S.; Kim, J .M.; Koh, W. S.
- Abstract
We made gene therapeutics for X-chronic granulomatous disease (CGD) by transducing murine bone marrow-derived stem cells with MT-gp91 retrovirus and evaluated possible toxicity in mice as a prerequisite for human clinical trials. Male C57BL/6 mice were injected intravenously with gene therapeutics for X-CGD twice at an interval of two weeks at 5 x 107 cells/kg and sacrificed 2 weeks after the last administration. Significant changes noted in gene therapeutics for X-CGD-treated animals were an increase in white blood cell counts and a slight decrease in albumin/globulin ratio. The red pulp hyperplasia in the spleen accompanied with an increase in organ weight was considered to result from the accumulation of gene therapeutics for X-CGD, bone marrow- derived stem cells, in the spleen. No anti-gp91 antibody was detected in the sera collected from the animals treated with gene therapeutics for X-CGD. No integration of gp91 DNA from retroviral vector was detected in chromosomal DNA of gonads in animals dosed with the test substance, indicaling no potential of genomic integration. In conclusion, the repeated dose of gene therapeutics for X-CGD exerted no toxicity. The splenic red pulp hyperplasia and the increase observed in white blood cell counts and in spleen weights were considered as pharmacological changes induced by the treatment.
- Subjects
TOXICOLOGY; CHRONIC granulomatous disease; STEM cells; LABORATORY rats; ALBUMINS; GLOBULINS; HYPERPLASIA; LEUCOCYTES; BLOOD plasma
- Publication
Human & Experimental Toxicology, 2008, Vol 27, Issue 5, p401
- ISSN
0960-3271
- Publication type
Article
- DOI
10.1177/0960327108094611