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- Title
Structural characterization of lipopeptides from Enterobacter sp. strain N18 reveals production of surfactin homologues.
- Authors
You, Jia; Yang, Shi‐Zhong; Mu, Bo‐Zhong
- Abstract
Lipopeptides are amphiphilic biosurfactants which contain fatty acid chains and peptide chains. A novel lipopeptide producer was isolated from soil samples and identified as Enterobacter sp. by using 16S rRNA gene sequencing. The surface tension of the cell-free broth decreased from 58.0 mN/m to 27.9 mN/m after the strain was incubated for 48 h at 37°C. The fatty acid part of the lipopeptide was elucidated by ESI-MS and GC-MS after methyl esterification, which showed that fatty acids were mixtures of n-C12, iso-C13, anteiso-C13, iso-C14, n-C14, iso-C15, anteiso-C15, iso-C16, n-C16 and iso-C17 β-hydroxy fatty acids. The peptide sequence, of which the composition of amino acids was determined by GC-MS with trimethylsilylation, was deduced to be N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C by using Q-TOF MS/MS. The fatty acid chain was bonded to the N-terminal of the peptide moiety, and a cyclic ring was constituted by the hydroxyl group of the fatty acid chain and the C-terminal. Practical applications The systematic characterization methods used in this article are helpful for structural analysis of those lipopeptide-like compounds. The information of Enterobacter-produced surfactin helps us to enlarge the family of surfactin-producers and expand the recognition of metabolites of Enterobacter spp. Cyclic lipopeptide produced by Enterobacter was characterized by a systematic method with the combination of ESI-MS, GC-MS and Q-TOF MS/MS. This was the first time that Enteobacter sp. was reported as surfactin-producer.
- Subjects
PEPTIDES; BIOSURFACTANTS; ENTEROBACTER; SURFACTIN; FATTY acids; HYDROXYL group
- Publication
European Journal of Lipid Science & Technology, 2015, Vol 117, Issue 6, p890
- ISSN
1438-7697
- Publication type
Article
- DOI
10.1002/ejlt.201400386