We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.
- Authors
Zhi Jiang; Huan Zhang; Chunliang Liu; Jun Yin; Shan Tong; Junxing Lv; Shaohua Wei; Shiliang Wu
- Abstract
β1,3-N-acetylglucosaminyltransferase (b3GnT8) and b3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary b1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer. They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine. However, whether b3GnT8 and b3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear. In our study, we detected the expression of b3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices. And b3GnT8, CD147, MMP2, and galectin3 were over-expressed in colorectal cancer tissues. We found that overexpression of b3GnT8 and b3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of b3GnT8 and b3GnT2 inhibited the invasive activity. Mechanistically, b3GnT8 and b3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells. Together, these results illustrate that the novel role and the molecular mechanism of b3GnT8 and b3GnT2 in promotion of colorectal cancer invasion. These results suggest that the potential use of b3GnT8 as a tumor target for the therapy of colorectal cancer.
- Subjects
COLON cancer; CANCER cells; CANCER invasiveness; GLYCAN structure; MATRIX metalloproteinases; IMMUNOHISTOCHEMISTRY
- Publication
Frontiers in Physiology, 2018, p1
- ISSN
1664-042X
- Publication type
Article
- DOI
10.3389/fphys.2018.00588