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- Title
An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response.
- Authors
Morita, Rihito; Hishinuma, Hisahiro; Ohyama, Hiromasa; Mega, Ryosuke; Ohta, Toshihiro; Nakagawa, Noriko; Agari, Yoshihiro; Fukui, Kenji; Shinkai, Akeo; Kuramitsu, Seiki; Masui, Ryoji
- Abstract
Alkylation is a type of stress that is fatal to cells. However, cells have various responses to alkylation. Alkyltransferase-like (ATL) protein is a novel protein involved in the repair of alkylated DNA; however, its repair mechanism at the molecular level is unclear. DNA microarray analysis revealed that the upregulation of 71 genes because of treatment with an alkylating agent N-methyl-N′-nitro-N-nitrosoguanidine was related to the presence of TTHA1564, the ATL protein from Thermus thermophilus HB8. Affinity chromatography showed a direct interaction of purified TTHA1564 with purified RNA polymerase holoenzyme. The amino acid sequence of TTHA1564 is homologous to that of the C-terminal domain of Ada protein, which acts as a transcriptional activator. These results suggest that TTHA1564 might act as a transcriptional regulator. The results of DNA microarray analysis also implied that the alkylating agent induced oxidation stress in addition to alkylation stress.
- Subjects
ALKYLATION; PETROLEUM refining; PROTEINS; DNA; GENES
- Publication
Journal of Biochemistry, 2011, Vol 150, Issue 3, p327
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvr052