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- Title
Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells.
- Authors
YU-YIN FU; YU-HUAN KANG; CONG-CONG SHEN; RUI-XUE WANG; LIN YU; XIN-YUE LI; DAN-DAN CUI; JIN-LIANG YANG; YU-QIN YAO; LAN-TU GOU
- Abstract
Human desumoylating isopeptidase 2 (DESI-2) is a member of the DESI family and contains a conserved PPPDE1 domain. Previous studies have demonstrated that DESI-2 overexpression may induce cell apoptosis. In the present study, differentially expressed genes were analyzed using a transcription microarray in DESI-2 overexpressing PANC-1 pancreatic cancer cells. A total of 45,033 genes were examined by microarray, which identified 1,766 upregulated and 1,643 downregulated genes. A series of altered signaling pathways were analyzed, in which certain essential signaling factors, including retinoid X receptor (RXR), BH3 interacting-domain death agonist, Ras homolog gene family member A (RhoA) and Rho-associated protein kinase, were further investigated at the protein level. The release of cytochrome c and the activation of caspase-3 were also detected by western blot analysis. Immunohistochemistry further revealed the expression features of RXR and RhoA in pancreatic ductal adenocarcinoma tissues with various DESI-2 expression levels. The results serve as a valuable reference for the further elucidation of the functions of DESI-2 in pancreatic cancer.
- Subjects
GENETIC overexpression; CELLULAR signal transduction; PEPTIDASE; CANCER patients; PANCREATIC cancer; CANCER cells; MICROARRAY technology
- Publication
Oncology Letters, 2016, Vol 12, Issue 6, p4677
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2016.5298