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- Title
Dynamics of the Interaction between <em>Entamoeba histolytica</em> and Components of the Immune Response II. On the Distinction of Surface-bound and Internalized Anti-amoeba Antibodies.
- Authors
Aust-Kettis, A.; Sundqvist, K.-G.
- Abstract
Tronhozoites of Entamoeba histolytica redistributed specific antibodies reacting to antigens on their surface. The presence of a second layer of anti-antibodies enhanced the redistribution of anti-amoeba antibodies but was not a prerequisite for this process. Microfluorometric measurements of the fluorescence intensity in different areas of the cell surface provided objective evidence for the antibody-induced redistribution. In order to distinguish surface-bound and internalized anti-amoeba antibodies, the trophozoites were fixed in PFA. This fixation made the cells impermeable to antibodies. Thus, after fixation in PFA at different times during the redistribution process surface-bound anti-amoeba antibodies labelled with one fluorochrome could be detected with anti-antibodies labelled with another fluorochrome. This approach showed that the amoeba, in addition to the redistribution, also internalized anti-amoeba antibodies.
- Subjects
ENTAMOEBA histolytica; IMMUNOGLOBULINS; ANTIGENS; FLUORESCENCE; RADIOACTIVITY; CELL membranes
- Publication
Scandinavian Journal of Immunology, 1980, Vol 12, Issue 5, p443
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00088.x