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- Title
A novel cardiac muscle-derived biomaterial reduces dyskinesia and postinfarct left ventricular remodeling in a mouse model of myocardial infarction.
- Authors
O'Connor, Daniel M.; Naresh, Nivedita K.; Piras, Bryan A.; Xu, Yaqin; Smith, Robert S.; Epstein, Frederick H.; Hossack, John A.; Ogle, Roy C.; French, Brent A.
- Abstract
Extracellular matrix ( ECM) degradation after myocardial infarction ( MI) leaves the myocardium structurally weakened and, as a result, susceptible to early infarct zone dyskinesia and left ventricular ( LV) remodeling. While various cellular and biomaterial preparations have been transplanted into the infarct zone in hopes of improving post- MI LV remodeling, an allogeneic cardiac muscle-derived ECM extract has yet to be developed and tested in the setting of reperfused MI. We sought to determine the effects of injecting a novel cardiac muscle-derived ECM into the infarct zone on early dyskinesia and LV remodeling in a mouse model of MI. Cardiac muscle ECM was extracted from frozen mouse heart tissue by a protocol that enriches for basement membrane constituents. The extract was injected into the infarct zone immediately after ischemia/reperfusion injury ( n = 6). Echocardiography was performed at baseline and at days 2, 7, 14, and 28 post- MI to assess 3D LV volumes and cardiac function, as compared to infarcted controls ( n = 9). Early infarct zone dyskinesia was measured on day 2 post- MI using a novel metric, the dyskinesia index. End-systolic volume was significantly reduced in the ECM-treated group compared to controls by day 14. Ejection fraction and stroke volume were also significantly improved in the ECM-treated group. ECM-treated hearts showed a significant ( P < 0.005) reduction in dyskinetic motion on day 2. Thus, using high-frequency ultrasound, it was shown that treatment with a cardiac-derived ECM preparation reduced early infarct zone dyskinesia and post- MI LV remodeling in a mouse model of reperfused MI.
- Subjects
MYOCARDIUM; LEFT ventricular hypertrophy; DYSKINESIAS; MYOCARDIAL infarction; EXTRACELLULAR matrix
- Publication
Physiological Reports, 2015, Vol 3, Issue 3, pn/a
- ISSN
2051-817X
- Publication type
Article
- DOI
10.14814/phy2.12351