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- Title
Phytochemical Constituents and Toxicity of Duguetia furfuracea Hydroalcoholic Extract in Drosophila melanogaster.
- Authors
de Araújo Pinho, Francisca Valéria Soares; da Silva, Gustavo Felipe; Macedo, Giulianna Echeverria; Muller, Katiane Raquel; Martins, Illana Kemmerich; Lausmann Ternes, Ana Paula; da Costa, José GalbertoMartins; Athayde, Margareth Linde; Boligon, Aline Augusti; Kamdem, Jean Paul; Franco, Jeferson Luis; de Menezes, Irwin Rose Alencar; Posser, Thaís
- Abstract
Duguetia furfuracea is frequently used as amedicinal plant in Brazil.However, studies have evidenced its cytotoxic, bactericide, and antitumor activities. In the present studywe aimed to evaluate the potential toxicity of hydroalcoholic leaves extracts of D. furfuracea (HEDF) in a Drosophila melanogaster model. Toxicity was assessed as changes in locomotor performance, mitochondrial activity, oxidative stress,MAPKs phosphorylation, and apoptosis induction after exposure to HEDF concentrations (1-50mg/mL) for 7 days. The phytoconstituents of the plant were screened for the presence of alkaloids, tannins, xanthones, chalcones, flavonoids, aurones, and phenolic acids. Exposure of adult flies to HEDF caused mitochondrial dysfunction, overproduction of ROS, and alterations in the activity of detoxifying enzymes GST, SOD and CAT. Induction of ERK phosphorylation and PARP cleavage was also observed, indicating occurrence of HEDF-induced cell stress and apoptotic cell death. In parallel, alterations in cholinesterase activity and impairments in negative geotaxis behavior were observed. Our study draws attention to the indiscriminate use of this plant by population and suggests oxidative stress as a major mechanism underlying its toxicity.
- Subjects
BRAZIL; BEHAVIORAL assessment; MITOCHONDRIAL physiology; PHENOL analysis; ENZYME metabolism; MEDICINAL plants; REACTIVE oxygen species; ALKALOIDS; ALTERNATIVE medicine; ANALYSIS of variance; APOPTOSIS; BIOLOGICAL assay; BIOPHYSICS; CELL death; DRUG toxicity; FLAVONOIDS; HIGH performance liquid chromatography; INSECTS; LEAVES; RESEARCH methodology; PHOSPHORYLATION; PROBABILITY theory; PROTEIN kinases; RESEARCH funding; STATISTICS; TANNINS; WESTERN immunoblotting; PHYTOCHEMICALS; PLANT extracts; DATA analysis; OXIDATIVE stress; DESCRIPTIVE statistics
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2014, Vol 2014, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2014/838101