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- Title
Selenium attenuates apoptosis and p-AMPK expressions in fluoride-induced NRK-52E cells.
- Authors
Gao, Jiping; Wang, Yu; Xu, Guoqiang; Wei, Jianing; Chang, Kai; Tian, Xiaolin; Liu, Maolin; Yan, Xiaoyan; Huo, Meijun; Song, Guohua
- Abstract
Fluoride is widely distributed in the environment, and excessive fluoride intake can induce cytotoxicity, DNA damage, and cell cycle changes in many tissues and organs, including the kidney. Accumulating evidence demonstrates that selenium (Se) administration ameliorates sodium fluoride (NaF)-induced kidney damage. However, the potentially beneficial effects of Se against NaF-induced cytotoxicity of the kidney and the underlying molecular mechanisms of this protection are not fully understood. At present, in this study, the normal rat kidney cell (NRK-52E) was used to investigate the potentially protective mechanism of Se against NaF-induced apoptosis, by using the methods of pathology, colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and Western blot. The experiment was designed with a control group, two NaF-treated groups (NaF, 5, 20 mg/L), two sodium selenite-treated groups (Na2SeO3, 17.1, 34.2 μg/L), and four Se + NaF-treated groups (Na2SeO3, 17.1, 34.2 μg/L; NaF, 5, 20 mg/L). The results indicate that selenium can attenuate apoptosis and AMPK phosphorylation in the NRK-52E cell induced with fluoride. These results imply that selenium is capable to modulate fluoride-induced NRK-52E cell apoptosis via regulating the expression levels of the proteins involved in mitochondrial pathway and changes in p-AMPK expressions may also be a key process in preventing fluorosis.
- Subjects
SELENIUM; SODIUM fluoride; DNA damage; FLOW cytometry; APOPTOSIS
- Publication
Environmental Science & Pollution Research, 2019, Vol 26, Issue 15, p15685
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1007/s11356-019-04855-2