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- Title
Induction of connective tissue growth factor expression by sphingosylphosphorylcholine in cultured human skin fibroblasts.
- Authors
Ming Ji Zhu; Chang Deok Kim; Yoo Bin Kwon; Kyung-Chae Kye; Yuan Yao Chen; Woong-Hee Lee; Sangkeun Lee; Jong Soon Lim; Young-Joon Seo; Ki-Beom Suhr; Jang-Kyu Park; Jeung-Hoon Lee
- Abstract
Zhu MJ, Kim CD, Kwon YB, Kye K-C, Chen YY, Lee W-H, Lee S, Lim JS, Seo Y-J, Suhr K-B, Park J-K, Lee J-H. Induction of connective tissue growth factor expression by sphingosylphosphorylcholine in cultured human skin fibroblasts. Sphingosylphosphorylcholine (SPC) is a bioactive sphingolipid metabolite that can enhance wound healing. In an effort to find downstream effectors of SPC, we performed microarray analysis and found that the expression of the gene for connective tissue growth factor (CTGF) was significantly affected in human skin fibroblasts cultured in vitro. Northern blot analysis showed that SPC markedly induced CTGF mRNA expression in a dose- and time-dependent manner. Consistent with this result, Western blot analysis also showed that SPC significantly induced the CTGF production. Pretreatment with cycloheximide did not prevent the CTGF induction by SPC, indicating that SPC stimulates CTGF mRNA expression without the increased synthesis of a regulatory protein. Inhibition by pretreatment with Y27632, but not by PD98059 (a mitogen-activated protein kinase 1/2 inhibitor) and LY294002 (a phosphatidylinositol 3-kinase inhibitor), indicated thatρ-kinase pathway was involved in SPC-induced CTGF expression. Together, these results reveal the potential importance of CTGF induction as a downstream event in SPC-induced cellular responses.
- Subjects
GROWTH factors; TISSUES; FIBROBLASTS; SKIN; METABOLITES; SPHINGOLIPIDS; WOUND healing
- Publication
Experimental Dermatology, 2005, Vol 14, Issue 7, p509
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/j.0906-6705.2005.00310.x