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- Title
Extracorporeal Photopheresis Improves Graft Survival in a Full-Mismatch Rat Model of Kidney Transplantation.
- Authors
Piñeiro, Gaston J.; Lazo-Rodriguez, Marta; Ventura-Aguiar, Pedro; Ramirez-Bajo, Maria J.; Banon-Maneus, Elisenda; Lozano, Miquel; Cid, Joan; Hierro-Garcia, Natalia; Cucchiari, David; Revuelta, Ignacio; Montagud-Marrahi, Enrique; Palou, Eduard; Bayés-Genís, Beatriu; Campistol, Josep M.; Diekmann, Fritz; Rovira, Jordi
- Abstract
Extracorporeal photopheresis (ECP) is an immunomodulatory therapy based on the infusion of autologous cellular products exposed to ultraviolet light (UV) in the presence of a photosensitizer. The study evaluates the ECP efficacy as induction therapy in a fullmismatch kidney transplant rat model. Dark Agouti to Lewis (DA-L) kidney transplant model has been established. ECP product was obtained from Lewis rat recipients after DA kidney graft transplantation (LewDA). Leukocytes of those LewDA rats were exposed to 8-methoxy psoralen, and illuminated with UV-A. The ECP doses assessed were 10 × 106 and 100 × 106 cells/time point. Lewis recipients received seven ECP infusions. DA-L model was characterized by the appearance of donor-specific antibodies (DSA) and kidney function deterioration from day three after kidney transplant. The dysfunction progressed rapidly until graft loss (6.1 ± 0.5 days). Tacrolimus at 0.25 mg/kg prolonged rat survival until 11.4 ± 0.7 days (p = 0.0004). In this context, the application of leukocytes from LewDA sensitized rats accelerated the rejection (8.7 ± 0.45, p = 0.0012), whereas ECP product at high dose extended kidney graft survival until 26.3 ± 7.3 days, reducing class I and II DSA in surviving rats. ECP treatment increases kidney graft survival in full-mismatch rat model of acute rejection and is a suitable immunomodulatory therapy to be explored in kidney transplantation.
- Subjects
KIDNEY transplantation; GRAFT survival; LABORATORY rats; ANIMAL disease models; KIDNEY physiology
- Publication
Transplant International, 2023, Vol 36, p1
- ISSN
0934-0874
- Publication type
Article
- DOI
10.3389/ti.2023.10840