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- Title
Improvement of Cucurbitacin B Content in Cucumis melo Pedicel Extracts by Biotransformation Using Recombinant β-Glucosidase.
- Authors
Jianfeng Mei; Xia Wu; Sujing Zheng; Xiang Chen; Zhuliang Huang; Yichun Wu
- Abstract
For the efficient biotransformation of cucurbitacin B 2-O-β-D-glucoside (CuBg) to cucurbitacin B (CuB) in Cucumis melo pedicel extracts, the β-glucosidase gene bglS—consisting of 1344 bp (447 amino acids) from Streptomyces sp. RW-2—was cloned and expressed in Escherichia coli BL21(DE3). The activity of recombinant β-glucosidase with p-nitrophenyl-β-D-glucoside (pNPG) as a substrate was 3.48 U/mL in a culture. Using the recombinant β-glucosidase for the biotransformation of C. melo pedicel extracts, CuBg was converted into CuB with a conversion rate of 87.6% when the concentration of CuBg was 0.973 g/L in a reaction mixtures. The concentration of CuB in C. melo pedicel extracts was improved from 13.6 to 20.2 g/L after biotransformation. The present study provides high-efficiency technology for the production of CuB from its glycoside by biotransformation.
- Subjects
CUCURBITACINS; MUSKMELON; GLUCOSIDASES; RECOMBINANT proteins; PLANT extracts
- Publication
Separations (2297-8739), 2021, Vol 8, Issue 9, p1
- ISSN
2297-8739
- Publication type
Article
- DOI
10.3390/separations8090138