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- Title
Changes in enzyme activities, soluble sugars and phenols during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth.
- Authors
Sarkar, A.; Rai, Ekta; Shah, Syed; Bouddha, Sulochna; Bansal, Y.; Ansari, S.
- Abstract
Changes in nitrate reductase, i.e. NR (E.C. 1.7.1.1) activity, peroxidase (E.C. 1.11.1.14) activity, soluble sugars and phenols were monitored at various time intervals from day 0 to 60 during in vitro adventitious shoot regeneration from leaflet explants of Albizia procera (Roxb.) Benth. The explants were incubated on semi-solid Murashige and Skoog medium supplemented with 2.6 g l Phytagel, 2.5% sucrose, 10 μM BAP, 1 μM NAA and 15 μM AgNO. NR activity, soluble sugars and phenols exhibited initial sharp rise on around day 20 followed by steep decline on day 25, whereas peroxidase activity peaked on day 50, highlighting significance of early input of nitrogen and energy and late emergence of lignification process for cellular differentiation and organization into adventitious shoot primordia. Morpho-anatomical changes in leaflets at various stages of in vitro adventitious shoot formation also followed the endogenous biochemical pattern.
- Subjects
EFFECT of nitrates on plants; PEROXIDASE; PHENOLS; REGENERATION (Botany); SUGARS; CELL differentiation; PLANT growing media
- Publication
New Forests, 2010, Vol 40, Issue 3, p323
- ISSN
0169-4286
- Publication type
Article
- DOI
10.1007/s11056-010-9202-3