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- Title
An Improved Colorimetric Assay for Bacterial Lipase in Nonfat Dry Milk.
- Authors
VERSAW, W. K.; CUPPETT, S. L.; WINTERS, D. D.; WILLIAMS, L. E.
- Abstract
A modification of McKellar and Cholette's (1986) calorimetric method for determining lipase activity in reconstituted non-fat dry milk was developed. The method uses the color reaction between Fast Blue BB and the β-naphthol (BN) enzymatically leaved from β-naphthyl caprylate (BNC) as a measure of lipase activity. In the modified method, a solvent system is used to clarify the sample rather than extracting the colored product. This modification allows measurement of total sample lipase by excluding centrifugation. In addition, the modified method has greater sensitivity with equal ease of use.
- Subjects
COLORIMETRY; BACTERIAL lipids; MILK; LIPASES; HYDROLASES
- Publication
Journal of Food Science (Wiley-Blackwell), 1989, Vol 54, Issue 6, p1557
- ISSN
0022-1147
- Publication type
Article
- DOI
10.1111/j.1365-2621.1989.tb05159.x