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- Title
Statistical optimization of production conditions of β-glucosidase from Bacillus stratosphericus strain SG9.
- Authors
Dutta, Soma; Shaik, Anver; Ganesh Kumar, C.; Kamal, Ahmed
- Abstract
The present study illustrates the optimization and characterization of β-glucosidase from a bacterial isolate, strain SG9. Sixty-eight different variables were first screened by one factor at a time method. The screened variable optimization was then performed by Plackett-Burman design followed by Box-Behnken response surface methodology. Thirty-one variables were screened, of which five variables were found to be significant. Box-Behnken design was then performed using the most significant variables, viz., esculin, KHPO and MgSO. The maximum enzyme activity was observed with an optimal medium composition of esculin (1.9 g/L), KHPO (0. 5 g/L) and MgSO (0.3 g/L) with a predicted value of 3392.01 IU. The maximum β-glucosidase production achieved was 3340 IU. The bacterial strain was identified by 16S rRNA gene sequence and biochemical characterization. The strain was identified as Bacillus stratosphericus and is a first report of its kind.
- Subjects
GLUCOSIDASES; ENZYMES; RIBOSOMAL RNA; BIOTECHNOLOGICAL process control; YEAST; FUNGI
- Publication
3 Biotech, 2017, Vol 7, Issue 3, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-017-0866-7