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- Title
Enzymatic preparation of L-α-glycerylphosphorylcholine in an aqueous medium.
- Authors
Zhang, Kangyi; Liu, Yuanfa; Wang, Xingguo
- Abstract
L-α-Glycerylphosphorylcholine ( L-α-GPC) was successfully prepared from phosphatidylcholine (PC) of food-grade soy lecithin powder using a novel enzymatic reaction in an aqueous medium. 94.5% yield of L-α-GPC was obtained under the optimal conditions of 55°C, 6.67 mg/mL substrate, 2 mM CaCl2, and 33.4 U/mL phospholipase A1 (Lecitase Ultra). L-α-GPC at 98% purity, 73.4% (wt%) recovery, and specific rotation ( $[{\rm \alpha }]_{{\rm D}}^{{\rm 20}} $) of −2.5° was achieved by silica gel column chromatography. Owing to its excellent catalytic efficiency, low cost, and ready availability, phospholipase A1 (Lecitase Ultra) provides a very satisfactory option for converting PC to L-α-GPC. Practical applications: L-α-Glycerylphosphorylcholine ( L-α-GPC) has been studied recently for its potential use as a supplement that may support neurological functions, but it is only found in trace amounts in nature. The present results indicate that Lecitase Ultra can be used for producing L-α-GPC from aqueous PC and suggest encouraging prospects for practical or industrial applications utilizing its notable catalytic performance, economy, and convenience.
- Publication
European Journal of Lipid Science & Technology, 2012, Vol 114, Issue 11, p1254
- ISSN
1438-7697
- Publication type
Article
- DOI
10.1002/ejlt.201100219