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- Title
Enzyme‐assisted development of biofunctional polyphenol‐enriched buckwheat protein: physicochemical properties, in vitro digestibility, and antioxidant activity.
- Authors
Chen, Xiao‐Wei; Chen, Ya‐Jun; Li, Jin‐Yu; Wang, Yong‐Hui; Yang, Xiao‐Quan
- Abstract
BACKGROUND During the last decade buckwheat was reported to have positive health effects. The present study investigated a high‐polyphenol buckwheat protein (Fagopyrum esculentum Moench) prepared by enzyme‐assisted processing, together with its physicochemical properties, in vitro digestibility, and antioxidant activity. RESULTS: Buckwheat protein prepared from the synergistic enzymatic action of α‐amylase and amyloglucosidase (E‐BWP) had much higher polyphenol content than buckwheat protein prepared by isoelectric precipitation (I‐BWP) or salt extraction (S‐BWP). Rutin degraded during the process, giving quercetin. The protein constituents and amino acid composition of E‐BWP were very similar to those of native buckwheat and were able to meet the WHO/FAO requirements for both children and adults. During in vitro digestion, E‐BWP showed anti‐digestive behavior with a nitrogen release that was lower than that of I‐BWP or S‐BWP. The positive effect of the polyphenol content of E‐BWP resulted in a higher 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) content and greater reducing activity. CONCLUSION: Buckwheat protein with high polyphenol content was successfully developed by enzyme‐assisted processing. It had a well‐balanced amino acid profile, antidigestive behavior, and high antioxidant activities. The results suggest that enzyme‐assisted processing is promising in the production of polyphenol‐enriched cereal protein, contributing higher functionality with good nutritional and antioxidant properties. © 2018 Society of Chemical Industry
- Subjects
BUCKWHEAT; POLYPHENOLS; OXIDANT status; GLUCOAMYLASE; ISOELECTRIC point
- Publication
Journal of the Science of Food & Agriculture, 2019, Vol 99, Issue 6, p3176
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.9534