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- Title
A generalized approach to detection of organophosphate resistance in mosquitoes.
- Authors
BROGDON, WILLIAM G.; BEACH, RAYMOND F.; BARBER, ANN M.; CORDON-ROSALES, CELIA
- Abstract
. Insecticide bioassays and biochemical microtitre assays were compared for detection of resistance to the organophosphate insecticides malathion and fenitrothion, using inbred laboratory strains of malaria vectors Anopheles albimanus Wiedemann, An.arabiensis Patton and An.stephensi Liston. With susceptible mosquitoes, the LT10o values determined from bioassays corresponded closely with times taken to abolish the activity of acetylcholinesterase activity in biochemical assays: approximately 2h for malathion and 3h for fenitrothion. Resistant strains of all three anophelines showed longer survival correlated with prolonged acetylcholinesterase activity. An.albimanus strains with insensitive acetylcholinesterase survived bioassays with discriminating doses of lh exposure to 5% malathion or 1% fenitrothion and were judged as resistant. It is concluded that enzyme-specific microassays provide a reliable means of detecting resistant individuals, with practical advantages over bioassays which do not reveal the resistance mechanism and require large numbers of healthy mosquitoes.
- Publication
Medical & Veterinary Entomology, 1992, Vol 6, Issue 2, p110
- ISSN
0269-283X
- Publication type
Article
- DOI
10.1111/j.1365-2915.1992.tb00585.x