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- Title
Discovery of a Protective <i>Rickettsia prowazekii</i> Antigen Recognized by CD8<sup>+</sup> T Cells, RP884, Using an <i>In Vivo</i> Screening Platform.
- Authors
Gazi, Michal; Caro-Gomez, Erika; Goez, Yenny; Cespedes, Maria A.; Hidalgo, Marylin; Correa, Paula; Valbuena, Gustavo
- Abstract
Rickettsia prowazekii has been tested for biological warfare due to the high mortality that it produces after aerosol transmission of very low numbers of rickettsiae. Epidemic typhus, the infection caused by these obligately intracellular bacteria, continues to be a threat because it is difficult to diagnose due to initial non-specific symptoms and the lack of commercial diagnostic tests that are sensitive and specific during the initial clinical presentation. A vaccine to prevent epidemic typhus would constitute an effective deterrent to the weaponization of R. prowazekii; however, an effective and safe vaccine is not currently available. Due to the cytoplasmic niche of Rickettsia, CD8+ T-cells are critical effectors of immunity; however, the identification of antigens recognized by these cells has not been systematically addressed. To help close this gap, we designed an antigen discovery strategy that uses cell-based vaccination with antigen presenting cells expressing microbe's proteins targeted to the MHC class I presentation pathway. We report the use of this method to discover a protective T-cell rickettsial antigen, RP884, among a test subset of rickettsial proteins.
- Subjects
RICKETTSIA prowazekii; CD80 antigen; T cells; BIOLOGICAL warfare; AEROSOL therapy; BACTERIAL proteins; CYTOPLASM
- Publication
PLoS ONE, 2013, Vol 8, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0076253