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- Title
Biological Activities and Cytotoxicity of Eperua oleifera Ducke Oil-resin.
- Authors
Gomes, Fernanda Torlania Alves; de Araújo Boleti, Ana Paula; Leandro, Lidiam M.; Squinello, Diego; Aranha, Ellen S. P.; Vasconcelos, Marne C.; Cos, Paul; Veiga-Junior, Valdir F.; Lima, Emerson Silva
- Abstract
Background: The oil-resin of Eperua oleifera Ducke has been used in popular medicine similarly to the copaiba oil (Copaifera spp.). Objective: This study aimed to investigate the effects of the acid fraction of E. oleifera oil-resin (AFEOR) on cell proliferation, collagen production in human fibroblasts, inhibition of metalloproteinases, and cytotoxicity against tumor cell lines. Materials and Methods: Acid fraction of E. oleifera was fractionated in the ion exchange column chromatography. Cytotoxicity and genotoxicity were evaluated by Alamar Blue® and Cometa assay. The inhibition of metalloproteinases was performed by zymography and Western blotting. Results: The predominant acidic diterpenes in the AFEOR were copalic and hardwickiic acids. AFEOR caused morphology alteration and decrease of proliferation at concentrations higher than 5 μg/mL. It also caused significant collagen proliferation in fibroblasts. It showed cytotoxicity against tumoral and nontumoral cell lines, with IC50 values ranging from 13 to 50 μg/mL, and a hemolytic activity with an IC50 value of 38.29 μg/mL. AFEOR inhibited collagenase activity, with an IC50 value of 46.64 μg/mL, and matrix metalloproteinase-2 (MMP)-2 and MMP-9 in HaCaT cells or MMP-1 expression in MRC-5 cells. AFEOR induced genotoxicity in MRC-5 cells with a DNA damage index between 40% and 60% when compared to the negative controls (0%-20%). Conclusion: For the first time, biological activities from oil-resin E. oleifera demonstrated ratifying somehow its popular use.
- Subjects
EPERUA; GUMS &; resins; COPAIBA; FIBROBLASTS; METALLOPROTEINASES; GENETIC toxicology
- Publication
Pharmacognosy Magazine, 2017, Vol 13, Issue 52, p542
- ISSN
0973-1296
- Publication type
Article
- DOI
10.4103/pm.pm_552_16