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- Title
Changes in Thiol-Disulfide Homeostasis of the Body to Surgical Trauma in Laparoscopic Cholecystectomy Patients.
- Authors
Polat, Murat; Ozcan, Onder; Sahan, Leyla; Üstündag-Budak, Yasemin; Alisik, Murat; Yilmaz, Nigar; Erel, Özcan; Üstündag-Budak, Yasemin; Erel, Özcan
- Abstract
<bold>Objective: </bold>We aimed to investigate the short-term effect of laparoscopic surgery on serum thiol-disulfide homeostasis levels as a marker of oxidant stress of surgical trauma in elective laparoscopic cholecystectomy patients.<bold>Materials and Methods: </bold>Venous blood samples were collected, and levels of native thiols, total thiols, and disulfides were determined with a novel automated assay. Total antioxidant capacity (measured as the ferric-reducing ability of plasma) and serum ischemia modified albumin, expressed as absorbance units assayed by the albumin cobalt binding test, were determined.<bold>Results: </bold>The major findings of the present study were that native thiol (283 ± 45 versus 241 ± 61 μmol/L), total thiol (313 ± 49 versus 263 ± 67 μmol/L), and disulfide (14.9 ± 4.6 versus 11.0 ± 6.1 μmol/L) levels were decreased significantly during operation and although they increased, they did not return to preoperation levels 24 hours after laparoscopic surgery compared to the levels at baseline. Disulfide/native thiol and disulfide/total thiol levels did not change during laparoscopic surgery.<bold>Conclusions: </bold>The decrease in plasma level of native and total thiol groups suggests impairment of the antioxidant capacity of plasma; however, the delicate balance between the different redox forms of thiols was maintained during surgery.
- Subjects
CHOLECYSTECTOMY; LAPAROSCOPIC surgery complications; DISULFIDES; SULFHYDRYL group; HOMEOSTASIS; OXIDIZING agents
- Publication
Journal of Laparoendoscopic & Advanced Surgical Techniques, 2016, Vol 26, Issue 12, p992
- ISSN
1092-6429
- Publication type
journal article
- DOI
10.1089/lap.2016.0381