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- Title
Development of stable film test for rapid estimation of blood or plasma 3-hydroxybutyrate.
- Authors
Harano, Yutaka; Aoki, Takahiko; Nakajima, Yuzuru; Kojima, Hideto; Kosugi, Keisuke; Harano, Aki; Hidaka, Hideki; Yamada, Shigeki; Uno, Shizuo; Shigeta, Yukio; Harano, Y; Aoki, T; Nakajima, Y; Kojima, H; Kosugi, K; Harano, A; Hidaka, H; Yamada, S; Uno, S; Shigeta, Y
- Abstract
A film test for the rapid detection of plasma/serum 3-hydroxybutyrate (3-OHB) has been developed. The film contains NAD, nitro blue tetrazolium, 3-OHB dehydrogenase, and diaphorase, and the surface is coated with modified biomembrane and can detect 50-1500 microM 3-OHB within 2-3 min. One drop or 50 microliters of plasma/serum or blood is applied to the film, and the violet color is read via reflectance meter after 2 min. Plasma/serum samples greater than 1500 microM 3-OHB can be measured by dilution with saline. In blood with 40% hematocrit, the color developed is 50% less than with plasma/serum, and this was adjusted in the reflectance meter. A good correlation (r = 0.99) was observed between results with automated and film methods and between visual methods and reflectance meter. In insulin-dependent diabetes mellitus, all 3 subjects with positive ketonuria (+ +), 8 of 12 subjects with mild ketonuria (+), and 7 of 25 subjects without ketonuria exhibited elevation of 3-OHB in blood greater than 200 microM. The results indicate that 3-OHB film is valuable not only in the emergency room for the differential diagnosis between ketoacidotic and nonketotic hypersomolar coma but also as a marker for insulin dependency, energy dependency on fatty acid compared with glucose, and metabolic control of diabetes.
- Publication
Diabetes Care, 1990, Vol 13, Issue 5, p522
- ISSN
0149-5992
- Publication type
journal article
- DOI
10.2337/diacare.13.5.522