We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
pGFPGUS Plus, a new binary vector for gene expression studies and optimising transformation systems in plants.
- Authors
Vickers, Claudia E.; Schenk, Peer M.; Dongxue Li; Mullineaux, Philip M.; Gresshoff, Peter M.
- Abstract
A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls.
- Subjects
GENE expression; REPORTER genes; GENETIC markers; TRANSGENIC plants; PLANT embryology; PLANT development; PLANT morphology; PLANT genetic transformation; PLANT biotechnology
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 11, p1793
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-007-9467-6