We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Stromal derived factor-1α in hippocampus radial glial cells in vitro regulates the migration of neural progenitor cells.
- Authors
Ding, Hui; Jin, Guo‐Hua; Zou, Lin‐Qing; Zhang, Xiao‐Qing; Li, Hao‐Ming; Tao, Xue‐Lei; Zhang, Xin‐Hua; Qin, Jian‐Bing; Tian, Mei‐Ling
- Abstract
Stromal derived factor-1α (SDF-1α), a critical chemokine that promotes cell homing to target tissues, was presumed to be involved in the traumatic brain injury cortex. In this study, we determined the expression of SDF-1α in the hippocampus after transection of the fimbria fornix (FF). Realtime PCR and ELISA showed that mRNA transcription and SDF-1α proteins increased significantly after FF transection. In vitro, the expression of SDF-1α in radial glial cells (RGCs) incubated with deafferented hippocampus extracts was observed to be greater than in those incubated with normal hippocampus extracts. The co-culture of neural progenitor cells (NPCs) and RGCs indicated that the extracts of deafferented hippocampus induced more NPCs migrating toward RGCs than the normal extracts. Suppression or overexpression of SDF-1α in RGCs markedly either decreased or increased, respectively, the migration of NPCs. These results suggest that after FF transection, SDF-1α in the deafferented hippocampus was upregulated and might play an important role in RGC induction of NPC migration; therefore, SDF-1α is a target for additional research for determining new therapy for brain injuries.
- Subjects
CHEMOKINES; STROMAL cells; HIPPOCAMPUS (Brain); NEUROGLIA; CELL migration; PROGENITOR cells
- Publication
Cell Biology International, 2015, Vol 39, Issue 6, p750
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10442