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- Title
Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present.
- Authors
Franco-Duarte, Ricardo; Černáková, Lucia; Kadam, Snehal; S. Kaushik, Karishma; Salehi, Bahare; Bevilacqua, Antonio; Corbo, Maria Rosaria; Antolak, Hubert; Dybka-Stępień, Katarzyna; Leszczewicz, Martyna; Relison Tintino, Saulo; Alexandrino de Souza, Veruska Cintia; Sharifi-Rad, Javad; Melo Coutinho, Henrique Douglas; Martins, Natália; Rodrigues, Célia F.
- Abstract
Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.
- Subjects
DETECTION of microorganisms; RIBOSOMAL RNA; POLYMERASE chain reaction; MICROORGANISM identification; NUCLEOTIDE sequence
- Publication
Microorganisms, 2019, Vol 7, Issue 5, p130
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms7050130