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- Title
Fast and Precise Protein Tracking Using Repeated Reversible Photoactivation.
- Authors
Chudakov, Dmitriy M.; Chepurnykh, Tatyana V.; Belousov, Vsevolod V.; Lukyanov, Sergey; Lukyanov, Konstantin A.
- Abstract
Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins’ movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report ‘protein rivers tracking’ technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.
- Subjects
PROTEIN engineering; PHOTOSENSITIZATION; MOLECULAR rotation of proteins; IMAGING systems in biology; PROTEIN analysis
- Publication
Traffic, 2006, Vol 7, Issue 10, p1304
- ISSN
1398-9219
- Publication type
Article
- DOI
10.1111/j.1600-0854.2006.00468.x