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- Title
Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF.
- Authors
Inukai, T; Sugita, K; Iijima, K; Goi, K; Tezuka, T; Kojika, S; Kagami, K; Mori, T; Kinoshita, A; Suzuki, T; O-Koyama, T; Nakazawa, S
- Abstract
We report a 20-month-old boy with acute lymphoblastic leukemia with the 11q23 translocation whose blasts markedly increased in peripheral blood after intravenous granulocyte colony-stimulating factor (G-CSF) administration, but disappeared after stopping G-CSF. The in vitro study showed that the leukemic cells separated from this patient expressed G-CSF receptor (G-CSFR) and an addition of G-CSF stimulated their proliferation by 3H-thymidine incorporation assay (stimulation index, 4.9). To clarify whether or not leukemic cells with 11q23 translocations generally express G-CSFR and show proliferative response to G-CSF, we performed the similar in vitro experiments using eight leukemic cell lines with 11q23 translocations. We found that all cell lines examined expressed G-CSFR (20-98%) and proliferation of seven leukemic cell lines was significantly enhanced in response to G-CSF (stimulation index >1.5 in five cell lines), suggesting a possible participation of the G-CSF/G-CSFR interaction in the process of growth regulation of leukemic cells with 11q23 translocations.
- Subjects
LYMPHOBLASTIC leukemia; CHROMOSOMAL translocation; GRANULOCYTE-colony stimulating factor; ANTINEOPLASTIC agents; LYMPHOBLASTIC leukemia treatment; B cells; CELL division; CELL lines; CELL receptors; CHROMOSOMES; DNA; ETOPOSIDE; GENE mapping; GRANULOCYTES; IMMUNOPHENOTYPING; CHRONIC myeloid leukemia; CYTARABINE; MITOXANTRONE; CANCER cell culture; BLOOD; THERAPEUTICS
- Publication
Leukemia (08876924), 1998, Vol 12, Issue 3, p382
- ISSN
0887-6924
- Publication type
journal article
- DOI
10.1038/sj.leu.2400951