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- Title
Determination of Flavan-3-ols by High-Performance Liquid Chromatography-Photodiode Array, Antioxidant Potential, Antimicrobial Activity, and Pharmacognostic Evaluation of Maytenus obtusifolia (Mart.) Leaves.
- Authors
Alves Fernandes de Araújo, Diego Igor; de Lima Arruda Fernandes, Anny Palloma; Vieira Pinheiro, Anderson Angel; Lopes Souto, Augusto; Aragão Sales, Kaio; Alves Fernandes de Araújo, Priscylla Rayama; Silva de Andrade, Rodrigo; Sobral da Silva, Marcelo
- Abstract
Background: Maytenus obtusifolia Mart. (Syn. Monteverdia obtusifolia Mart. Biral), Celastraceae, is a species located on the coast of Brazil, which is commonly used as anti-inflammatory and on the treatment of gastric ulcers. Objectives: The aim of this study consists of the determination of quality control parameters of the plant material, as well as to develop, validate high-performance liquid chromatography-photodiode array methodology for the quantification of its analytical markers and evaluate the antioxidant potential and antimicrobial activity of the extract. Materials and Methods: The plant material and ethanolic crude extract (ECE) were evaluated through pharmacopoeial and nonpharmacopoeial methods. Anti-oxidant assay was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Minimum inhibitory concentration was used to evaluate antimicrobial activity assay. Results: The assays have provided the following results: granulometry (d50 0.82 mm), moisture content (6.41%-9.03%), pH of aqueous solution (5.41), apparent bulk density (0.41 mg/mL), heavy metals (<10 ppm), total ash (5.20%), and sulfated ash content (6.36%). Total flavonoid (2.14%; 6.24%), tannin (6.51%; 17.85%), and phenolic content (41.95; 134.76 mg AG/g) regarding leaves powder and its ECE, respectively. The validated method was specific, linear, precise, accurate, and robust. The content of (-)-Epigallocatechin, (+)-Catechin and (-)-Epicatechin on the ECE was 1.00%, 0.40%, and 0.33%, respectively. The anti-oxidant activity was evaluated by DPPH assay and have presented an IC50 at 18.01 µg/mL. In addition, the most important antimicrobial activity of the ECE was against Aspergillus flavus (MIC at 128 µg/mL). Conclusion: These data have demonstrated to be very useful toward the analysis and pharmacognostic quality control of M. obtusifolia Mart.
- Subjects
BRAZIL; MAYTENUS; ANTIOXIDANTS; QUALITY control; ASPERGILLUS flavus; LIQUIDS; MALTODEXTRIN; TANNINS
- Publication
Pharmacognosy Research, 2020, Vol 12, Issue 3, p313
- ISSN
0976-4836
- Publication type
Article
- DOI
10.4103/pr.pr_9_20