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- Title
Role of integrin-linked kinase in osteosarcoma progression.
- Authors
Rhee, Seung Hwan; Han, Ilkyu; Lee, Mi Ra; Cho, Hwan Seong; Oh, Joo Han; Kim, Han ‐ Soo
- Abstract
ABSTRACT Although integrin-linked kinase (ILK) has been suggested to play a role in the tumorigenesis of a number of human epithelial carcinomas, little is known of its role in musculoskeletal sarcoma. The authors studied ILK expression by immunohistochemistry using osteosarcoma prechemotherapy specimens from 56 patients, and investigated the prognostic implications of the findings obtained. It was found that ILK overexpression was significantly correlated with the presence of distant metastasis ( p = 0.008) and that it was an independent prognostic factor for both poor overall survival and poor event-free survival ( p = 0.015 and 0.010, respectively). During a transfection experiment conducted by transfecting osteosarcoma cells with ILK siRNA, VEGF concentrations were measured using an ELISA kit, and then compared with those of untransfected controls to evaluate its angiogenic effects. In addition, apoptotic percentages were measured by Annexin-V flow cytometry, and invasive properties were evaluated by measuring the numbers of non-migrating cells in a Boyden chamber. It was found that ILK downregulation significantly decreased angiogenesis, increased apoptosis, and decreased invasiveness of osteosarcoma cells. These results show that ILK is a promising prognostic factor in osteosarcoma and a novel potential therapeutic target for the treatment of osteosarcoma. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1668-1675, 2013
- Subjects
INTEGRIN-linked kinase; OSTEOSARCOMA; CANCER invasiveness; NEOPLASTIC cell transformation; MUSCULOSKELETAL system; APOPTOSIS; IMMUNOHISTOCHEMISTRY; TUMORS
- Publication
Journal of Orthopaedic Research, 2013, Vol 31, Issue 10, p1668
- ISSN
0736-0266
- Publication type
Article
- DOI
10.1002/jor.22409