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- Title
Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.
- Authors
Certo, Michael T; Gwiazda, Kamila S; Kuhar, Ryan; Sather, Blythe; Curinga, Gabrielle; Mandt, Tyler; Brault, Michelle; Lambert, Abigail R; Baxter, Sarah K; Jacoby, Kyle; Ryu, Byoung Y; Kiem, Hans-Peter; Gouble, Agnes; Paques, Frederic; Rawlings, David J; Scharenberg, Andrew M
- Abstract
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.
- Subjects
ENDONUCLEASES; GENES; DNA repair; DNA damage; MUTAGENESIS; GENOMES
- Publication
Nature Methods, 2012, Vol 9, Issue 10, p973
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2177