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- Title
Effect of DAPK methylation and Bcl2 over expression on risk of recurrence in Transitional Cell Carcinoma of bladder.
- Authors
Zafarghandi MOTLAGH, Fatemeh; Arjomand FARD, Nazanin; MAJIDIZADEH, Tayebeh; ASGARI, Mojgan; ABOlHASANI, Maryam; SHAHSHAHANIPOUR, Majid; KAMALI, Koosha; GHADAKZADEH, Saleh; HOSSEINI, Seyed Ali
- Abstract
Although, several factors dictate bladder cancer progression and patient survival, mechanisms for bladder carcinogenesis and the development of bladder cancer remain unclear. Aberrant methylation of CpG islands and deregulation of apoptosis, which are found in early stages of carcinogenesis are one of the specifications of human cancers. We aimed to investigate expression of the Bcl2 and methylation status of DAPK, in determining the risk for bladder cancer recurrence. DAPK promoter hypermethylation was analyzed in 80 histologically confirmed incidents of transitional Cell Carcinoma (TCC) of the bladder specimens and 80 healthy samples by MS-PCR. DAPK methylation was found in 36.25% of tissue samples of TCC. the incidence of DAPK hypermethylation showed significant association with an increase in age (p=0.01), recurrence (p= 0.0001), and smoking status (p=0.03) but no association was found with other clinicopathological parameters. overall, 21.15% of samples displayed cytoplasmic staining of Bcl2 protein expression. however, 9.6% of patients had DAPK promoter hypermethylation and Bcl2 over expression with poor prognosis and metastasis. our findings demonstrated that hypermethylation of the DAPK gene and Bcl2 expression leads to TCC recurrence and may have clinical implications for TCC progno sis and therapy.
- Subjects
BLADDER cancer; CANCER invasiveness; METHYLATION; APOPTOSIS; CARCINOGENESIS; TRANSITIONAL cell carcinoma; CANCER prognosis
- Publication
Journal of Biological Research, 2013, Vol 19, p56
- ISSN
1790-045X
- Publication type
Article