We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Allelic dilution obscures detection of a biologically significant resistance mutation in EGFR-amplified lung cancer.
- Authors
Engelman, Jeffrey A.; Mukohara, Toru; Zejnullahu, Kreshnik; Lifshits, Eugene; Borrás, Ana M.; Gale, Christopher-Michael; Naumov, George N.; Yeap, Beow Y.; Jarrell, Emily; Sun, Jason; Tracy, Sean; Xiaojun Zhao; Heymach, John V.; Johnson, Bruce E.; Cantley, Lewis C.; Jänne, Pasi A.; Borrás, Ana M; Zhao, Xiaojun; Jänne, Pasi A
- Abstract
EGFR is frequently mutated and amplified in lung adenocarcinomas sensitive to EGFR inhibitors gefitinib and erlotinib. A secondary mutation, T790M, has been associated with acquired resistance but has not been shown to be sufficient to render EGFR mutant/amplified lung cancers resistant to EGFR inhibitors. We created a model for studying acquired resistance to gefitinib by prolonged exposure of a gefitinib-sensitive lung carcinoma cell line (H3255; EGFR mutated and amplified) to gefitinib in vitro. The resulting resistant cell line acquired a T790M mutation in a small fraction of the amplified alleles that was undetected by direct sequencing and identified only by a highly sensitive HPLC-based technique. In gefitinib-sensitive lung cancer cells with EGFR mutations and amplifications, exogenous introduction of EGFR T790M effectively conferred resistance to gefitinib and continued ErbB-3/PI3K/Akt signaling when in cis to an activating mutation. Moreover, continued activation of PI3K signaling by the PIK3CA oncogenic mutant, p110alpha E545K, was sufficient to abrogate gefitinib-induced apoptosis. These findings suggest that allelic dilution of biologically significant resistance mutations may go undetected by direct sequencing in cancers with amplified oncogenes and that restoration of PI3K activation via either a T790M mutation or other mechanisms can provide resistance to gefitinib.
- Subjects
EPIDERMAL growth factor; LUNG cancer; ADENOCARCINOMA; ANTINEOPLASTIC agents; GENETIC mutation; CELL lines; CELL receptors; APOPTOSIS; ALLELES; ANIMAL experimentation; CELL physiology; COMPARATIVE studies; DRUG resistance in cancer cells; GENE amplification; GENES; GENETIC techniques; HETEROCYCLIC compounds; LUNG tumors; RESEARCH methodology; MEDICAL cooperation; MICE; NUCLEOTIDES; PHOSPHORYLATION; PHOSPHOTRANSFERASES; RESEARCH; RESEARCH funding; TRANSFERASES; EVALUATION research; CHEMICAL inhibitors; PROTEIN kinase inhibitors; PHARMACODYNAMICS; THERAPEUTICS
- Publication
Journal of Clinical Investigation, 2006, Vol 116, Issue 10, p2695
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI28656