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- Title
Production of odd chain polyunsaturated fatty acids by Mortierella fungi.
- Authors
Shimizu, Sakayu; Kawashima, Hiroshi; Akimoto, Kengo; Shinmen, Yoshifumi; Yamada, Hideaki
- Abstract
A soil isolate, Mortierella alpina 1S-4, was found to show high production of odd chain polyunsaturated fatty acids (PUFAs) among various arachidonic acid-producing Mortierella strains tested. The fungus mainly accumulated 5,8,11,M-cis-nonadecatetraenoic acid. With 5% n-hepta-decane and 1% yeast extract as growth substrates, the amount of C:4 acid accumulated reached 44.4 mg/g dry mycelia (0.68 mg/mL of culture broth). This value accounted for 11.2% of the total fatty acids in the extracted lipids from mycelia, and odd chain fatty acids comprised over 95% of the total mycelial fatty acids. The addition of sesamin, a specific inhibitor of A5 desaturation, caused an increase in C acid and an accompanying decrease in C acid. On the other hand, species of Mortierella that could not produce C-20 PUFAs accumulated C-17 acids, but no C-19 PUFAs, when grown with fatty substrates with an odd chain skeleton. The odd chain PUFAs were distributed in both neutral and polar lipids. The biosynthetic route to C acid was presumed to mimic the n-6 route to arachidonic acid as follows: C → C→ C→ C → C → C acids.
- Publication
Journal of the American Oil Chemists' Society (JAOCS), 1991, Vol 68, Issue 4, p254
- ISSN
0003-021X
- Publication type
Article
- DOI
10.1007/BF02657620