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- Title
Nitrated Fatty Acids Modulate the Physical Properties of Model Membranes and the Structure of Transmembrane Proteins.
- Authors
Franz, Johannes; Bereau, Tristan; Pannwitt, Stefanie; Anbazhagan, Veerappan; Lehr, Alexander; Nubbemeyer, Udo; Dietz, Ulrich; Bonn, Mischa; Weidner, Tobias; Schneider, Dirk
- Abstract
Nitrated fatty acids (NO2-FAs) act as anti-inflammatory signal mediators, albeit the molecular mechanisms behind NO2-FAs' influence on diverse metabolic and signaling pathways in inflamed tissues are essentially elusive. Here, we combine fluorescence measurements with surface-specific sum frequency generation vibrational spectroscopy and coarse-grained computer simulations to demonstrate that NO2-FAs alter lipid organization by accumulation at the membrane-water interface. As the function of membrane proteins strongly depends on both, protein structure as well as membrane properties, we consecutively follow the structural dynamics of an integral membrane protein in presence of NO2-FAs. Based on our results, we suggest a molecular mechanism of the NO2-FA in vivo activity: Driven by the NO2-FA-induced lipid layer reorganization, the structure and function of membrane-associated (signaling) proteins is indirectly affected.
- Subjects
NITRO compounds; MEMBRANE proteins; ANTI-inflammatory agents; PROTEIN structure; FLUORESCENCE spectroscopy; INTERFACES (Physical sciences)
- Publication
Chemistry - A European Journal, 2017, Vol 23, Issue 40, p9690
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201702041