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- Title
Triple Resonance MAS NMR with (<sup>13</sup>C, <sup>15</sup>N) Labelled Molecules: Reduced Dimensionality Data Acquisition Via <sup>13</sup>C-<sup>15</sup>N Heteronuclear Two-Spin Coherence Transfer Pathways.
- Authors
Leppert, Jörg; Heise, Bert; Ohlenschlöger, Oliver; Görlach, Matthias; Ramachandran, Ramadurai
- Abstract
A reduced dimensionality magic angle spinning solid-state NMR experimental protocol for obtaining chemical shift correlation spectra of dipolar coupled nuclei in uniformly (13C, 15N) labelled biological systems is described and demonstrated. The method involves a mapping of the evolution frequencies of heteronuclear 13C-15N zero- and double-quantum coherences. In comparison to a reduced dimensionality procedure involving the simultaneous incrementation of two single-quantum chemical shift evolution periods, the approach described here could be potentially advantageous for minimising the heat dissipated in the probe by high power 1H decoupling in experiments requiring long t1 acquisition times.
- Subjects
RESONANCE; SPIN labels; SOLID state chemistry; NUCLEAR magnetic resonance; CHARGE transfer; BIOPHYSICAL labeling
- Publication
Journal of Biomolecular NMR, 2004, Vol 28, Issue 2, p185
- ISSN
0925-2738
- Publication type
Article
- DOI
10.1023/B:JNMR.0000013834.36262.79