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- Title
Organic Anion Transporting Polypeptide 3A1 (OATP3A1)-Gated Bio-Orthogonal Labeling of Intracellular Proteins.
- Authors
Németh, Krisztina; László, Zsófia; Biró, Adrienn; Szatmári, Ágnes; Cserép, Gergely B.; Várady, György; Bakos, Éva; Özvegy-Laczka, Csilla; Kele, Péter
- Abstract
Organic anion transporting polypeptides (OATPs) were found to readily deliver membrane impermeable, tetrazine bearing fluorescent probes into cells. This feature was explored in OATP3A1 conditioned bio-orthogonal labeling schemes of various intracellular proteins in live cells. Confocal microscopy and super-resolution microscopy (STED) studies have shown that highly specific and efficient staining of the selected intracellular proteins can be achieved with the otherwise non-permeable probes when OATP3A1 is present in the cell membrane of cells. Such a transport protein linked bio-orthogonal labeling scheme is believed to be useful in OATP3A1 activity-controlled protein expression studies in the future.
- Subjects
CARRIER proteins; CONFOCAL microscopy; FLUORESCENT probes; PROTEINS; PROTEIN transport; ORGANIC anion transporters
- Publication
Molecules, 2023, Vol 28, Issue 6, p2521
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28062521