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- Title
Improved cell growth and biosynthesis of glycolic acid by overexpression of membrane-bound pyridine nucleotide transhydrogenase.
- Authors
Cabulong, Rhudith B.; Valdehuesa, Kris Niño G.; Bañares, Angelo B.; Ramos, Kristine Rose M.; Nisola, Grace M.; Lee, Won-Keun; Chung, Wook-Jin
- Abstract
The non-conventional D-xylose metabolism called the Dahms pathway which only requires the expression of at least three enzymes to produce pyruvate and glycolaldehyde has been previously engineered in Escherichia coli. Strains that rely on this pathway exhibit lower growth rates which were initially attributed to the perturbed redox homeostasis as evidenced by the lower intracellular NADPH concentrations during exponential growth phase. NADPH-regenerating systems were then tested to restore the redox homeostasis. The membrane-bound pyridine nucleotide transhydrogenase, PntAB, was overexpressed and resulted to a significant increase in biomass and glycolic acid titer and yield. Furthermore, expression of PntAB in an optimized glycolic acid-producing strain improved the growth and product titer significantly. This work demonstrated that compensating for the NADPH demand can be achieved by overexpression of PntAB in E. coli strains assimilating D-xylose through the Dahms pathway. Consequently, increase in biomass accumulation and product concentration was also observed.
- Subjects
CELL growth; XYLOSE; METABOLISM; ENZYMES; PYRUVATES; GLYCOLALDEHYDE; ESCHERICHIA coli
- Publication
Journal of Industrial Microbiology & Biotechnology, 2019, Vol 46, Issue 2, p159
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-018-2117-2