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- Title
New polymorphisms in the interleukin-10 gene – relationships to myocardial infarction.
- Authors
Donger, C.; Georges, J-L.; Nicaud, V.; Morrison, C.; Evans, A.; Kee, F.; Arveiler, D.; Tiret, L.; Cambien, F.
- Abstract
Interleukin-10 (IL-10) is a cytokine with anti-inflammatory and B-cell-stimulating activity. IL-10 is expressed in human atherosclerotic plaques and recent studies have shown the involvement of IL-10 in the atherosclerotic process. Therefore, we hypothesized that polymorphisms in the IL-10 gene might be associated with a predisposition to coronary heart disease. To identify new polymorphisms in the human IL-10 gene, the entire coding sequence and the 3′ flanking sequence of the gene were screened by polymerase chain reaction–single strand conformation polymorphism (PCR‐SSCR) followed by sequencing. The polymorphisms identified, and three others which have been previously described in the promoter region of the IL-10 gene (G-1082A, C-819T, C-592A), were then investigated in the ECTIM Study, a large population-based case–control study of myocardial infarction. Four new polymorphisms were identified: one in exon 1 (G+78/ex1A), which predicts a Glycine to Arginine change at position 15 in the putative signal peptide of the protein, two in the intron 3 (C+19/in3T, T+953/in3C) and one in the 3′ flanking region (C+117T). All the IL-10 polymorphisms were in complete or nearly complete pairwise linkage disequilibrium. No case–control difference was found in genotype or allele frequencies for any of the polymorphisms. Our results suggest that IL-10 polymorphisms are not associated with an increased risk of myocardial infarction.
- Subjects
MYOCARDIAL infarction; INTERLEUKIN-10; GENETIC polymorphisms; GENETICS
- Publication
European Journal of Clinical Investigation, 2001, Vol 31, Issue 1, p9
- ISSN
0014-2972
- Publication type
Article
- DOI
10.1046/j.1365-2362.2001.00754.x