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- Title
Measurement of Kallikrein Activity in Urine of Rats and Man Using a Chromogenic Tripeptide Substrate.
- Authors
Bönner, G.; Marin-Grez, M.
- Abstract
Urinary kallikrein activity in rats and man was measured by an amidolytic assay using the chromogenic tripeptide/P-valyl-leucyl-arginine-p-nitroanilide (S 2266). The sensitivity of the assay permits the measurement of 3.5 to 250 mKU of purified porcine pancreatic kallikrein. The intraassay coefficient of variation was 1.6 ± 0.3 % and the interassay coefficient of variation 7.4 ± 0.9%. No changes in kallikrein activity were found in urine samples kept for 7 days at 4 °C or 14 months at -20 °C. Repeated freezing and thawing reduced kallikrein activity. Kallikrein activity of the same urine samples was also estimated by means of a bradykinin radioimmunoassay: which determines the kinins released during incubation of urine with partially purified dog kininogen (kininogenase activity). The urinary kallikrein activity assayed by means of these two methods showed a high correlation with coefficients of r = 0.9314 for rat urine (p < 0.001) and r = 0.8002 for human urine (p < 0.001).
- Publication
Clinical Chemistry & Laboratory Medicine, 1981, Vol 19, Issue 3, p165
- ISSN
1434-6621
- Publication type
Article