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- Title
Retinoblastoma Protein Prevents Staurosporine-Induced Cell Death in a Retinoblastoma-Defective Human Glioma Cell Line.
- Authors
Yamasaki, Fumiyuki; Kajiwara, Yoshinori; Hama, Seiji; Murakami, Taro; Hidaka, Toshikazu; Saito, Taiichi; Yoshioka, Hiroyuki; Sugiyama, Kazuhiko; Arita, Kazunori; Kurisu, Kaoru
- Abstract
Objective: To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine. Methods: Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing Rb, p16 or p21 genes in human glioma cell lines. Results: The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the Rb gene, thus decreasing the death rate of these cells. Conclusions: These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects. Copyright © 2007 S. Karger AG, Basel
- Publication
Pathobiology, 2007, Vol 74, Issue 1, p22
- ISSN
1015-2008
- Publication type
Article
- DOI
10.1159/000101048