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- Title
Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development.
- Authors
Rybiczka-Tešulov, Mateja; Garritsen, Oxana; Venø, Morten T.; Wieg, Laura; Dijk, Roland van; Rahimi, Karim; Gomes-Duarte, Andreia; Wit, Marina de; van de Haar, Lieke L.; Michels, Lars; van Kronenburg, Nicky C. H.; van der Meer, Christiaan; Kjems, Jørgen; Vangoor, Vamshidhar R.; Pasterkamp, R. Jeroen
- Abstract
Midbrain dopamine (mDA) neurons play an essential role in cognitive and motor behaviours and are linked to different brain disorders. However, the molecular mechanisms underlying their development, and in particular the role of non-coding RNAs (ncRNAs), remain incompletely understood. Here, we establish the transcriptomic landscape and alternative splicing patterns of circular RNAs (circRNAs) at key developmental timepoints in mouse mDA neurons in vivo using fluorescence-activated cell sorting followed by short- and long-read RNA sequencing. In situ hybridisation shows expression of several circRNAs during early mDA neuron development and post-transcriptional silencing unveils roles for different circRNAs in regulating mDA neuron morphology. Finally, in utero electroporation and time-lapse imaging implicate circRmst, a circRNA with widespread morphological effects, in the migration of developing mDA neurons in vivo. Together, these data for the first time suggest a functional role for circRNAs in developing mDA neurons and characterise poorly defined aspects of mDA neuron development. The function of circular RNAs in the brain remains poorly understood. Here the authors identify a role for circular RNAs in the control of neuron morphology and migration in the developing midbrain dopamine system.
- Subjects
ALTERNATIVE RNA splicing; CIRCULAR RNA; NEURON development; NON-coding RNA; TRIGONOMETRIC functions; DOPAMINE receptors
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-51041-1