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- Title
Gold nanoparticles coated with carbosilane dendrons in protein sample preparation.
- Authors
Vásquez-Villanueva, Romy; Peña-González, Cornelia E.; Sánchez-Nieves, Javier; de la Mata, F. Javier; Marina, M. Luisa; García, M. Concepción
- Abstract
The feasibility of using carbosilane dendronized gold nanoparticles (GNPs) for protein sample preparation was evaluated. Three different dendrons with three different generations (1G, 2G, and 3G) were employed to modify the GNPs, viz. sulfonate terminated (STC-GNPs), carboxylate terminated (CTC-GNPs), and trimethylammonium terminated (ATC-GNPs) dendrons. The synthesis of the CTC-GNP is described. The other dendronized GNPs were synthesized using previously described routes. Bovine serum albumin, lysozyme, and myoglobin were employed to study the potential of GNPs to interact with proteins. The interaction between the GNPs and the proteins was evaluated using fluorescence spectroscopy and polyacrylamide gel electrophoresis. The CTC-GNPs and STC-GNPs under acidic and neutral conditions, respectively, promoted the establishment of electrostatic interactions with positively charged proteins. Proteins from 10 to 75 kDa molecular weights interacted with GNPs at protein: nanoparticle ratios of 1:0.25. The GNPs were applied to the extraction of proteins from a peach seed. In the authors' perception, the method is a clean alternative to established extraction methods based on the use of organic or polluting chemicals.
- Subjects
GOLD nanoparticles; GOLD coatings; CHEMICAL sample preparation; POLYACRYLAMIDE gel electrophoresis; SILANE; MOLECULAR weights; FLUORESCENCE spectroscopy
- Publication
Microchimica Acta, 2019, Vol 186, Issue 8, pN.PAG
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-019-3587-2