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- Title
Novel Antibody Capture Assay for Paraffin-Embedded Tissue Detects Wide-Ranging Amyloid Beta and Paired Helical Filament-Tau Accumulation in Cognitively Normal Older Adults.
- Authors
Postupna, Nadia; Rose, Shannon E.; Bird, Thomas D.; Gonzalez-Cuyar, Luis F.; Sonnen, Joshua A.; Larson, Eric B.; Keene, C. Dirk; Montine, Thomas J.
- Abstract
Quantifying antigens in formalin-fixed tissue is challenging and limits investigation in population-based studies of brain aging. To address this major limitation, we have developed a new technique that we call 'Histelide': immunohistochemistry (HIST-) and enzyme-linked immunosorbent assay (ELISA) (-EL-) performed on a glass slide (-IDE). We validated Histelide in sections of prefrontal cortex from 20 selected cases: 12 subjects with clinically and neuropathologically diagnosed Alzheimer's disease (AD), either autosomal dominant or late-onset forms, and 8 clinical and neuropathologic controls. AD cases had significantly increased amyloid beta (Aβ) peptide and paired helical filament- (PHF-) tau per area of neocortex that was proteinase K-sensitive, and significantly decreased amount of synaptophysin. We next investigated prefrontal cortex from 81 consecutive cases of high-cognitive performers from the Adult Changes in Thought (ACT) study, a population-based study of brain aging and incident dementia. As expected, latent AD was common in this group; however, our results quantified widely individually varying levels of Aβ peptides and PHF-tau among these high-cognitive performers. This novel approach obtains quantitative data from population-based studies, and our initial studies with high-cognitive performers provide important quantitative insights into latent AD that should help guide expectations from neuroimaging and prevention studies.
- Subjects
IMMUNOASSAY; IMMUNOGLOBULINS; AMYLOID beta-protein; IMMUNOHISTOCHEMISTRY; ENZYME-linked immunosorbent assay; ALZHEIMER'S disease; TAU proteins; COGNITION in old age
- Publication
Brain Pathology, 2012, Vol 22, Issue 4, p472
- ISSN
1015-6305
- Publication type
Article
- DOI
10.1111/j.1750-3639.2011.00542.x