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- Title
Identification of two subpopulations of purified human blood B cells, CD27<sup>−</sup> CD23<sup>+</sup> and CD27<sup>high</sup> CD80<sup>+</sup>, that strongly express cell surface Toll-like receptor 9 and secrete high levels of interleukin-6.
- Authors
Cognasse, Fabrice; Hamzeh-Cognasse, Hind; Lafarge, Sandrine; Chavarin, Patricia; Pozzetto, Bruno; Richard, Yolande; Garraud, Olivier
- Abstract
B-cell expression of certain Toll-like receptors (TLRs) is important in linking innate and adaptive immune responses in normal and pathological conditions. The expression of TLR9 plays a role in the recognition of conserved pathogen motifs in a manner that is dependent on B-cell localization, deduced from B-cell phenotype. The nature of TLR9 function is unclear. A first step in unravelling the function of this pattern recognition receptor is to discover the precise nature of the cell types that express TLR9. This study used three-colour flow cytometry to characterize the B lymphocytes from human peripheral blood mononuclear cells (PBMCs) that express TLR9 on the surface. We sorted TLR9-positive B and non-B cells from the PBMC population and detected TLR9 expression on naïve and memory B cells. Moreover, we identified two discrete subpopulations of B cells: CD19+ CD27− CD23+ cells and CD19+ CD27high CD80+ cells. These subpopulations expressed high levels of membrane TLR9 and exhibited a strong in vitro response to binding a relevant CpG motif by secreting high levels of interleukin-6 (compared to controls). Our finding that this pattern recognition receptor is expressed on a variety of cell subsets adds to the current understanding of the functional complexity of B-cell membrane TLR9.
- Subjects
BLOOD cells; INTERLEUKINS; B cells; PHENOTYPES; LYMPHOCYTES
- Publication
Immunology, 2008, Vol 125, Issue 3, p430
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1111/j.1365-2567.2008.02844.x