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- Title
Hippocampal Long-Term Potentiation Is Supported by Presynaptic and Postsynaptic Tyrosine Receptor Kinase B-Mediated Phospholipase Cγ Signaling.
- Authors
Gärtner, Annette; Polnau, Dorit G.; Staiger, Volker; Sciarretta, Carla; Minichiello, Liliana; Thoenen, Hans; Bonhoeffer, Tobias; Korte, Martin
- Abstract
Neurotrophins have been shown to play a critical role in activity-dependent synaptic plasticity such as long-term potentiation (LTP) in the hippocampus. Although the role of brain-derived neurotrophic factor (BDNF) and its tyrosine kinase receptor [tyrosine receptor kinase B (TrkB)] is well documented, it still remains unresolved whether presynaptic or postsynaptic activation of TrkB is involved in the induction of LTP. To address this question, we locally and specifically interfered with a downstream target of the TrkB receptor, phospholipase Cγ(PLCγ). We prevented PLCγ signaling by overexpression of the PLCγ pleckstrin homology (PH) domain with a Sindbis virus vector. The isolated PH domain has an inhibitory effect and thereby blocks endogenous PLCγ signaling and consequently also IP3 production. Surprisingly, concurrent presynaptic and postsynaptic blockade of PLCγ signaling was required to reduce LTP to levels comparable with those in TrkB and BDNF knock-out mice. Blockade of presynaptic or postsynaptic signaling alone did not result in a significant reduction of LTP.
- Subjects
SYNAPSES; HIPPOCAMPUS (Brain); NEUROTROPHINS; TYROSINE; PREPARATIVE layer chromatography
- Publication
Journal of Neuroscience, 2006, Vol 26, Issue 13, p3496
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.3792-05.2006