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- Title
Bovine β-lactoglobulin acts as an acid-resistant drug carrier by exploiting its diverse binding regions.
- Authors
Barbiroli, Alberto; Beringhelli, Tiziana; Bonomi, Francesco; Donghi, Daniela; Ferranti, Pasquale; Galliano, Monica; Iametti, Stefania; Maggioni, Daniela; Rasmussen, Patrizia; Scanu, Sandra; Vilardo, Maria Caterina
- Abstract
Binding of fluorine-containing drugs to bovine β-lactoglobulin, the most abundant whey protein in bovine milk, was investigated by means of 19F NMR and mass spectrometry. The stoichiometry of the binding and its stability in acidic medium, where β-lactoglobulin is folded and stable, were also studied, along with competition from molecules that can be regarded as analogs of physiological ligands to bovine β-lactoglobulin. Conditional binding data were combined with protein structural information derived from circular dichroism and limited proteolysis studies. Spectroscopic techniques were also used to assess whether the bound drugs stabilize the protein structure against denaturation by chaotropes or temperature at various pH values. The results obtained provide evidence for the presence of multiple binding regions on the protein, with a specific and different affinity for structurally different classes of hydrophobic drugs and, more generally, that bovine β-lactoglobulin can bind and protect against low pH values various classes of drugs of pharmaceutical relevance.
- Subjects
LACTOGLOBULINS; DRUG carriers; GLOBULINS; WHEY; MILK yield
- Publication
Biological Chemistry, 2010, Vol 391, Issue 1, p21
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/BC.2010.008