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- Title
Utilization of Medium-Chain Triglycerides and Octanoic Acid by Cultured Human Fibroblasts and Lymphoblasts.
- Authors
LEMMEN, Christa; STUHLER, Karl; LEMMEN, Peter; WOLFRAM, Günther
- Abstract
Cultured human fibroblasts and lymphoblasts were incubated with emulsions containing 14C-trioctanoin or 14C-tripalmitin. Both cell types were able to hydrolyse the medium-chain triglyceride but not the long-chain triglyceride to the corresponding fatty acids. At the end of a 3 days incubation period, 25-30% of the initial amount of 10 nmol/ml trioctanoin were present as triglyceride. The observed hydrolysis seems to be mediated by an esterase secreted into the culture medium, as was shown by the use of cell-conditioned medium. CO2 production from octanoic acid was below 2 nmol per mg protein and day, demonstrating that these cells have a low capacity to use this substrate for their energy metabolism.
- Publication
Biological Chemistry, 1988, Vol 369, Issue 2, p1267
- ISSN
1431-6730
- Publication type
Article